NOT ALL HIGH-SCORES are ESEs !
- The presence of a high-score motif in a sequence does NOT necessarily identify
that sequence as an exonic splicing enhancer in its native context.
For example, a nearby silencer element may prevent the SR protein
- The default threshold values are based on statistical analysis and empirical
data, but they are still somewhat arbitrary. Any refinements
or updates will be incorporated as they become available
- There is at best only a rough correlation between numerical scores and ESE
activity of high-score motifs. For example, the maximum score
is not necessarily the most effective ESE.
- The score values of ESEs corresponding to different SR proteins cannot be compared
to each other.
- The program currently searches the ESE motifs
corresponding to four SR proteins. There are several other SR
proteins for which the ESE motifs have not yet been identified,
at least by the strategy used here.
- The ESE motifs were
identified using human SR proteins. Their relevance to other
species depends on the extent of conservation of each SR